Elisa Assays, imunofluorescenční zařízení a PCR testy
Neočekávané falešně pozitivní sazby
Peter14 května, 20210 Comments
Neočekávané falešně pozitivní sazby v pediatrické sérologii SARS-CoV-2 s použitím testu ELIM IgG Anti-SARS-CoV-2 ELISA IgG
Aims: Serologic assay efficiency research for extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in pediatric populations are missing, and few seroprevalence research have routinely included orthogonal testing to enhance accuracy.
Strategies: Remnant serum samples for routine bloodwork from 2,338 pediatric sufferers at UPMC Kids’s Hospital of Pittsburgh had been assessed utilizing the EUROIMMUN Anti-SARS-CoV-2 ELISA IgG (EuroIGG) assay. Reactive circumstances with enough quantity had been additionally examined utilizing Three further business assays.
Outcomes: Eighty-five specimens had been reactive in response to the EuroIGG, yielding 3.64% (95% confidence interval [CI], 2.91%-4.48%) seropositivity, of which 73 specimens had enough remaining quantity for affirmation by orthogonal testing. General, 19.18% (95% CI, 10.18%-28.18%) of samples had been constructive on a second and/or third orthogonal assay. This 80.82% false positivity charge is disproportionate to the anticipated false positivity charge of 50% given our pediatric inhabitants prevalence and assay efficiency.
Conclusions: In pediatric populations, false-positive SARS-CoV-2 serology could also be extra widespread than assay and prevalence parameters would predict, and additional research are wanted to determine the efficiency of SARS-CoV-2 serology in youngsters.
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MAD reagent 1, 30 ul of 100 x MAD reagent 2 and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of ADP using a standard black 384-well plate.
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MGD reagent 1, 30 ul of 100 x MGD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of GDP using a standard black 384-well plate.
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MUD reagent 1, 30 ul of 100 x MUD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of UDP using a standard black 384-well plate.
Description: This product includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x NTPs (ATP, UTP, GTP, CTP) and 700 µl of 10 x fluorescence dye. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format. The assay kit includes all reagents except the enzyme.
Description: This product includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x mM NTPs (ATP, UTP, GTP, CTP), 700 µl of 10 x fluorescence dye and 50 µl of 50 x SARS-CoV-2 RdRp. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format.
Description: The Human LEP ELISA kit is designed to detect and quantify the level of Human LEP in cell culture supernatant, serum, plasma and tissue. This kit is for research use only and not intended for diagnostic purposes.
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: EGTT-Scavenger (cat# EGTT-SCVG) is a chemical reagent used for sample treatment with the GSH/GSSG Assay Kit (cat#: EGTT-100). Necessary for cell lysate samples used in the EGTT-100 assay kit. Key Features: Ready-to-use 500µL solution, sufficient for 100 tests with EGTT-100 assay kit.
NucView 488 and MitoView 633 Apoptosis Assay Kit (100 assays)
Description: This product includes 400 ul of 10 x Buffer, 105 ul of 250 ug/ml relaxed DNA, 220 ul of 10 mM ATP, 550 ul of 5 x Gel loading buffer and 25 ul of 2000 nM E.coli gyrase.
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitative measurement of Human leptin in serum, plasma and other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Description of target: Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 10 pg/mL
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Klinický význam IgM a IgA anti-pneumokokových polysacharidových testů ELISA u pacientů s podezřením na nedostatek protilátek
Not like IgG pneumococcal polysaccharide(PnPS)-antibodies, PnPS IgA and IgM-antibodies are usually not routinely decided for the evaluation of immunocompetence. It’s not but recognized whether or not an remoted lack of ability to mount a standard IgM or IgA-PnPS response ought to be thought-about a related major antibody deficiency (PAD). We studied the scientific relevance of anti-PnPS IgM and IgA-assays in sufferers with suspected major immunodeficiency in a big educating hospital in ‘s-Hertogenbosch, the Netherlands. Serotype-specific-PnPS IgG-assays had been carried out, subsequently, 23-valent-PnPS IgG-assays (anti-PnPS IgG-assays), and later anti-PnPS IgA and IgM-assays had been carried out in archived materials (240 sufferers; 304 samples). 11/65 pre-immunisation and 6/10 post-immunisation samples from good responders to PnPS-serotype-specific IgG-testing, had decreased anti-PnPS IgA and/or IgM-titres. Of those, three pre-immunisation and no post-immunisation samples had been from sufferers beforehand categorised as ‘no PAD’. Dedication of anti-PnPS IgA and IgM along with anti-PnPS IgG didn’t scale back the necessity for serotype-specific-PnPS IgG-testing to evaluate immunocompetence (ROC-analysis of post-immunisation samples: anti-PnPS IgA+IgG AUC 0.80(95%-CI 0.63-0.97); anti-PnPS IgM+IgG AUC 0.80(95%-CI 0.62-0.98); anti-PnPS IgA+IgG+IgM AUC 0.71(95%-CI 0.51-0.91); anti-PnPS IgG AUC 0.93(95%-CI 0.85-1.00)).
Our knowledge present that sufferers, categorised as having an intact antibody response primarily based on measurement of serotype-specific-PnPS IgG, nonetheless can show impaired anti-PnPS IgM- and IgA-responses, and that the extra measurement of anti-PnPS IgA and IgM couldn’t scale back the necessity for serotype-specific IgG-testing. Future research are wanted to research the scientific relevance of potential ‘particular IgA- or IgM-antibody deficiency’ in sufferers with recurrent airway infections in whom no PAD might be identified in response to the present definitions.
ePCL Electrospun Microfibrous Layers for Immune Assays: Delicate ELISA for the Detection of Serum Protileads In opposition to HPV16 E7 Oncoprotein
Human papillomavirus (HPV) sort 16 is the etiologic agent of greater than 50% anal/cervical cancers and about 20% oropharyngeal cancers. HPV16 E6 and E7 oncogenes favor the transformation and are important for sustaining the remodeled standing. Serum anti-E6 and anti-E7 antibodies seem to have prognostic significance for HPV-associated cancers. Nonetheless, a lot of the earlier makes an attempt to determine diagnostic instruments primarily based on serum detection of E6 and/or E7 antibodies have been unsuccessful, primarily because of the low accuracy of utilized checks.
This paper reviews on a feasibility research to show the likelihood to simply immobilize HPV16 E7 onto electrospun substrates for software in diagnostic instruments. On this research, poly(ε-caprolactone) electrospun scaffolds (known as ePCL) are used to offer a microstructured substrate with a excessive surface-to-volume ratio, able to binding E7 proteins when used for enzyme-linked immunosorbent assay (ELISA) checks. ePCL functionalized with E7 exhibited superior properties in comparison with normal polystyrene plates, rising the detection sign from serum antibodies by 5-6 occasions. Evaluation of the serum samples from mice immunized with HPV16 E7 DNA vaccine confirmed increased effectivity of this new anti-E7 ePCL-ELISA check vs management in E7-specific antibody detection. As well as, ePCL-E7-ELISA is ready with a comparatively low quantity of antigen, reducing the manufacturing prices.
Mapování epitopů toxinu záškrtu a vývoj diagnostického testu specifického professional ELISA
Background: The diphtheria toxoid antigen is a serious element in pediatric and booster mixture vaccines and is understood to lift a protecting humoral immune response upon vaccination. Though antibodies are thought-about important for diphtheria safety, little is understood in regards to the antigenic determinants that preserve humoral immunity. Strategies:One-hundred and twelve 15 mer peptides protecting the complete sequence of diphtheria toxin (DTx) protein had been ready by SPOT synthesis.
The immunoreactivity of membrane-bound peptides with sera from mice immunized with a triple DTP vaccine allowed mapping of steady B-cell epitopes, topological research, multiantigen peptide (MAP) synthesis, and Enzyme-Linked Immunosorbent Assay (ELISA) improvement. Outcomes: Twenty epitopes had been recognized, with two being within the sign peptide, 5 in the catalytic area (CD), seven within the HBFT area, and 5 within the receptor-binding area (RBD).
Two 17 mer (CB/Tx-2/12 and CB/DTx-4-13) derived biepitope peptides linked by a Gly-Gly spacer had been chemically synthesized. The peptides had been used as antigens to coat ELISA plates and assayed with human (huVS) and mice vaccinated sera (miVS) for in vitro analysis of diphtheria. The assay proved to be extremely delicate (99.96%) and particular (100%) for huVS and miVS and, in comparison with a business ELISA check, demonstrated a excessive efficiency. Conclusions: Our work displayed the entire image of the linear B cell IgG response epitope of the DTx answerable for the protecting impact and demonstrated enough specificity and eligibility for part IIB research of some epitopes to develop new and quick diagnostic assays.
Korelace automatické chemiluminiscenční metody s enzymově vázanými imunosorbentními testy (ELISA) u protilátek u rekonvalescentních vzorků plazmy COVID-19: vývoj rychlých a nákladově efektivních semikvantitativních diagnostických metod
Background: We investigated the utility of an automatic chemiluminescent SARS-CoV-2 IgG antibody assay platform in quantifying the quantity of binding antibodies current in donated convalescent plasma.
Strategies: A complete of 179 convalescent plasma items had been analyzed for the presence of SARS-CoV-2 IgG antibodies utilizing the Beckman-Coulter chemiluminescent immunoassay (CLIA) platform. The equipment-derived numerical values (S/Co ratio) had been recorded. Aliquots from the identical items had been subjected to enzyme-linked immunosorbent assay (ELISA) that detects IgG antibodies towards the receptor-binding area (RBD) of the SARS-CoV-2 S1 protein. The connection between ELISA titers and CLIA S/Co values was analyzed utilizing linear regression and receiver working traits (ROC) curve.
Outcomes: Twenty-one samples (11.7%) had S/Co values of lower than 1.Zero and had been deemed destructive for antibodies and convalescent plasma had S/Co values between >1.Zero and 5.0 (70/179, 39.1%). Fifteen items (8.4%) had destructive ELISA titer. Nearly all of the items (95/179. 53.1%) had titers ≥1:1024. The sensitivities of ELISA to CLIA had been comparable (90.5% vs 88.3%, respectively; p=0.18). There was constructive linear correlation between CLIA S/Co values and ELISA IgG titer (Rho = 0.75; Spearman’s rank = 0.82, p-value = <0.0001). The settlement between the 2 strategies was truthful, with a κ index of 0.2741. Utilizing the ROC evaluation, we recognized a CLIA S/Co cutoff worth of 8.2, which supplies a sensitivity of 90% and a specificity of 82% in predicting a titer dilution of ≥1:1024.
Conclusion: The utility of automated antibody detection methods may be prolonged from merely a screening methodology to a semi-quantitative and quantitative practical antibody evaluation. CLIA S/Co values can be utilized to reliably estimate the ELISA antibody titer. Incorporation of chemiluminescent-based strategies can present fast, cost-effective technique of figuring out anti-SARS-CoV-2 antibody titers in donated plasma to be used within the therapy of COVID-19 an infection.
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.
Description: Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of plasma proteins with chlorinated oxidants such as chloramines or hypochlorous acid. AOPP levels are elevated in patients with renal complications, diabetes mellitus, and atherosclerosis, as well as HIV-positive patients. Our OxiSelect AOPP Assay Kit provides a convenient method to assess oxidative stress. The kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control.
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MAD reagent 1, 30 ul of 100 x MAD reagent 2 and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of ADP using a standard black 384-well plate.
Description: This product includes 1800 ul of 10 x Buffer, 150 ul of 100 x MAD reagent 1, 150 ul of 100 x MAD reagent 2 and 1500 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 500 samples of ADP using a standard black 384-well plate.
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MGD reagent 1, 30 ul of 100 x MGD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of GDP using a standard black 384-well plate.
Description: This product includes 1800 ul of 10 x Buffer, 150 ul of 100 x MGD reagent 1, 150 ul of 100 x MGD reagent 2 and 1500 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 500 samples of GDP using a standard black 384-well plate.
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MUD reagent 1, 30 ul of 100 x MUD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of UDP using a standard black 384-well plate.
Description: This product includes 1800 ul of 10 x Buffer, 150 ul of 100 x MUD reagent 1, 150 ul of 100 x MUD reagent 2 and 1500 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 500 samples of UDP using a standard black 384-well plate.
Description: A competitive ELISA for quantitative measurement of Human adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Human Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human globular Adiponectin (gAcrp30) ELISA Kit, 96 tests, Quantitative
Description: A competitive ELISA for quantitative measurement of Rabbit Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Adiponectin (ADP) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Dog Adiponectin (ADP) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Gallus Adiponectin (ADP) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Adiponectin (ADP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Adiponectin (ADP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rabbit Adiponectin (ADP) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Adiponectin Receptor 2 (ADIPOR2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Adiponectin Receptor 1 (ADIPOR1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Human Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Adiponectin (ADP)Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Description: A competitive ELISA for quantitative measurement of Rabbit High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey High molecular weight Adiponectin ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine High molecular weight Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.
Description: Quantitative sandwich ELISA kit for measuring Human adiponectin, ADP in samples from serum, plasma, cell culture supernates, tissue homogenates, urine. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative sandwich ELISA kit for measuring Human adiponectin, ADP in samples from serum, plasma, cell culture supernates, tissue homogenates, urine. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Description of target: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 60 pg/ml
Description: A competitive ELISA for quantitative measurement of Human adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Lymphocyte Antigen 96 (LY96) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Human High Molecular Weight Adiponectin(HMW Adiponectin)ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Human High Molecular Weight Adiponectin (HMW Adiponectin) in samples from serum, plasma, cell culture supernates, tissue homogenates, urine. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
