Quality level: 300

Biological source: goat

Antibody form: affinity isolated antibody

Antibody product type: secondary antibodies

Clone: polyclonal

Species reactivity: Rabbit

Should not react with: human

Application(s)

Direct ELISA: 1: 30,000 using 5 μg / ml rabbit IgG for coating and OPD substrate
immunohistochemistry (paraffin-embedded sections, formalin-fixed): 1: 200
Western blot (chemiluminescent): 1: 80,000-1: 160,000 using the detection of β-actin in the total cellular extract of HeLa cells (5-10 μg / mL)

Conjugate: peroxidase conjugate

Sent in: dry ice

Related categories: Secondary antibodies

General description

The goat anti-rabbit IgG peroxidase conjugate associates with all rabbit Ig. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). In addition, the antibody is adsorbed with human IgG to reduce background staining with human samples.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and helps mainly in immune defense. IgG is an important class of immunoglobulin. Immunoglobulin G (IgG) is involved in type II and type III hypersensitivity. Rabbit IgG is divided into five classes: IgM, IgG, IgA, IgD, and IgE. It has only one IgG variant with an inner weighted disulfide bridge. It aids in opsonization, complement fixation, and antibody-dependent cell-mediated cytotoxicity.

Specificity

The specificity of anti-rabbit IgG peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP.

Immunogen

Purified rabbit IgG

Request

• Anti-rabbit IgG (whole molecule): The peroxidase antibody has been used in Western blotting, immunofluorescence staining, immunochemistry, and immunoprecipitations.
• Peroxidase-conjugated goat anti-rabbit IgG can be used for direct ELISA and immunohistochemistry.
• Peroxidase-conjugated goat anti-rabbit IgG antibody was used for Western blot analysis of Cos 7 cell lysates at 1: 2000 dilution and yeast lysates after protein carbonylation assays at 1: dilution. 5000.
• Goat-produced rabbit anti-IgG (whole molecule) -peroxidase antibody has been used as a secondary antibody during immunofluorescence staining.
  Coimmunoprecipitation and Western blot analysis of C33A cell lysates was performed using HRP-conjugated goat anti-rabbit IgG as the secondary antibody.

Biochemical / Physiological Actions

IgGs (immunoglobulins) modulate various immune responses and primarily help in immune defense. Immunoglobulin G (IgG) is involved in type II and type III hypersensitivity. It aids in opsonization, complement fixation, and antibody-dependent cell-mediated cytotoxicity.

Physical form

The solution in 0.01 M phosphate-buffered saline, pH 7.4, containing 0.05% MIT

Preparation note

Prepared by the two-step glutaraldehyde method.

Storage and stability

For continuous use, store at 2-8 ° C for up to one month. For long-term storage, the solution can be frozen in working aliquots. Repeated freezing and thawing, or storing in “frost-free” freezers is not recommended. If slight cloudiness occurs after prolonged storage, rinse the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product (s), our products are intended for research use only and are not to be used for any other purpose, including but not limited to commercial uses. unauthorized, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.