Vyhodnocení ELISA, imunofluorescence a stanovení laterálního průtoku pro SARS-CoV-2 sérologické testy Background: The SARS-CoV-2 outbreak has emerged at the end of 2019. Aside from the detection of viral genome with specific RT-PCR, there is a growing need for reliable determination of the serological status. We aimed at evaluating five SARS-CoV-2 serology assays. Methods: An in-house immunofluorescence assay […]
Fotooxidací indukovaný systém zesílení
Fotooxidací indukovaný systém zesílení fluorescence pro ultracitlivý enzymový imunosorbentní test (ELISA) This report suggests a method of enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). The PIFA utilized autocatalytic photooxidation of the chemifluorescent substrate, 10-acetyl 3,7-dihydroxyphenoxazine (ADHP, Amplex Red) to amplify the fluorescent product resorufin, initially oxidized by horse radish peroxidase (HRP). […]
Srovnání myší Toxoplasma gondii
Srovnání myší Toxoplasma gondii a antigenů odvozených z buněčné kultury v testu ELISA Toxoplasmosis is an infectious illness brought on by the coccidian parasite Toxoplasma gondii. Prognosis relies on serological strategies with detection of particular IgG and IgM antibodies. The current research was carried out to check the sensitivity and specificity of soluble antigen […]
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