Srovnání myší Toxoplasma gondii

0 Comments
r-web

Srovnání myší Toxoplasma gondii a antigenů odvozených z buněčné kultury v testu ELISA

 

Toxoplasmosis is an infectious illness brought on by the coccidian parasite Toxoplasma gondii. Prognosis relies on serological strategies with detection of particular IgG and IgM antibodies. The current research was carried out to check the sensitivity and specificity of soluble antigen of T. gondii, RH pressure obtained from mice and cell tradition in ELISA methodology. Tachyzoites of T. gondii, RH pressure that inoculated in mice peritoneum have been collected. On the identical time, tachyzoites have been harvested from HeLa cell tradition that contaminated with the parasite.

Soluble antigen was ready and ELISA methodology carried out on 100 serum samples that have been collected from totally different laboratories in Tehran, Iran. Business Trinity package was used as gold commonplace. The sensitivity and specificity of T.gondii soluble antigen have been larger in antigens that obtained from cell tradition compared with mice peritoneum. T. gondii cell tradition derived antigen has excessive sensitivity and specificity in ELISA take a look at.

r-web
r-web

FcR Blocking Reagent

FCR-4ML 400 test
EUR 282.7
Description: FcR

FcR blocking Reagent

20-abx290024
  • Ask for price
  • Ask for price
  • 200 tests
  • 400 tests

Mouse IgG Blocking Reagent

30R-AH071 20 mg
EUR 219
Description: Mouse IgG (H + L) Blocking Reagent

PhosphoBlocker™ Blocking Reagent

AKR-103 1L
EUR 265

PhosphoBlocker™ Blocking Reagent

AKR-104 4L
EUR 630

Human-to-Human Blocking Reagent

HTH008 8 ml
EUR 20.73

Human-to-Human Blocking Reagent

HTH015 15 ml
EUR 26.53

Human-to-Human Blocking Reagent

HTH100 100 ml
EUR 156.85

Mouse-to-Mouse Blocking Reagent

MTM008 8 ml
EUR 24.43

Mouse-to-Mouse Blocking Reagent

MTM015 15 ml
EUR 30.85

Mouse-to-Mouse Blocking Reagent

MTM125 125 ml
EUR 250.71

Mouse-to-Mouse Blocking Reagent

MTM500 500 ml
EUR 623.56

Rabbit-to-Rabbit Blocking Reagent

RTR008 8 ml
EUR 24.43

Rabbit-to-Rabbit Blocking Reagent

RTR015 15 ml
EUR 30.85

Rabbit-to-Rabbit Blocking Reagent

RTR125 125 ml
EUR 250.71

Rabbit-to-Rabbit Blocking Reagent

RTR500 500 ml
EUR 623.56

CytoWatchâ„¢ QZ100 Monocyte Blocking Reagent

37000-100Test 100 Test
EUR 79
Description: Some dye-labeled fluorescent antibody conjugates used in cell surface staining of live cells often exhibit non-specific binding in monocytes and macrophages.

CytoWatchâ„¢ QZ100 Monocyte Blocking Reagent

37001-500Test 500 Test
EUR 412
Description: Some dye-labeled fluorescent antibody conjugates used in cell surface staining of live cells often exhibit non-specific binding in monocytes and macrophages.

Amersham ECL Advance Blocking Reagent 40g - EACH

RPN418 EACH
EUR 117.45

BLOCK ACE Reagent

GWB-Q00190 20 x 4g Ask for price

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers - EACH

5730 EACH
EUR 1074.6

ELISA BSA BLOCK Reagent

GWB-Q00212 100 ml Ask for price

ELISA BSA BLOCK Reagent

GWB-Q00213 500 ml Ask for price

ELISA BSA BLOCK Reagent

GWB-Q00214 1000 ml Ask for price

Block-Free ELISA Reagent

M4102-250 each
EUR 542.4

Block-Free ELISA Reagent

M4102-50 each
EUR 176.4

Block-Free ELISA Reagent (Protein-Free)

M4101-250 each
EUR 548.4

Block-Free ELISA Reagent (Protein-Free)

M4101-50 each
EUR 183.6

OOSA09542-80G - BLOCK ACE Accessory Reagent

OOSA09542-80G 20x4g
EUR 679

OOSA09551-1L - ELISA BSA BLOCK Accessory Reagent

OOSA09551-1L 1000ml
EUR 549

EP Reagent Biuret Reagent - 100ML

1011601 1L
EUR 59.4

OOSA09549-100ML - ELISA BSA BLOCK Accessory Reagent

OOSA09549-100ML 100ml
EUR 159

OOSA09550-500ML - ELISA BSA BLOCK Accessory Reagent

OOSA09550-500ML 500ml
EUR 359

EP Reagent Iodoplatinate Reagent - 200ML

1046300 200ML
EUR 502.2

EP Reagent Molybdovanadic Reagent - 100ML

1056700 100ML
EUR 59.4

EP Reagent Methoxyphenylacetic Reagent - 100ML

1053601 100ML
EUR 490.05

EP Reagent Phosphomolybdotungstic Reagent - 100ML

1065000 100ML
EUR 226.8

Gordon-McLeod Reagent (Oxidase reagent)

R026-100ML 1 unit
EUR 7.76
Description: Gordon-McLeod Reagent (Oxidase reagent)

EP Reagent Sulfomolybdic Reagent R3 - 1L

1086500 1L
EUR 398.25

N.N.C.D.-reagent

N632003 1g
EUR 1200
Description: 6035-19-4

MTS Reagent

2808-1000 each
EUR 1188

MTS Reagent

2808-250 each
EUR 438

MTT Reagent

2809-1G each
EUR 216

MTT Reagent

2809-5G each
EUR 652.8

BOP reagent

5-02141 25g Ask for price

BOP reagent

5-02142 100g Ask for price

BOP reagent

A7015-100000 100 g
EUR 104
Description: Peptide coupling reagent

BOP reagent

A7015-25000 25 g
EUR 44
Description: Peptide coupling reagent

TDA Reagent

R036-10ML 1 unit
EUR 5.22
Description: TDA Reagent

PYR Reagent

R043-10ML 1 unit
EUR 12.02
Description: PYR Reagent

Reagent pack

A11-100 1 unit
EUR 123

pNPP Reagent

GWB-Q00238 100 ml Ask for price

pNPP Reagent

GWB-Q00239 500 ml Ask for price

100 BlotBot Reagent Reservoirs

RRVR-100 100pack
EUR 212.4
Description: BlotBot reagent reservoirs will allow dispensing of unique reagents during processing. Pack of 100.

500 BlotBot Reagent Reservoirs

RRVR-500 500pack
EUR 640.8
Description: BlotBot reagent reservoirs will allow dispensing of unique reagents tray during processing. Pack of 500.

Traut's Reagent

2330-1000 each
EUR 418.8

Traut's Reagent

2330-500 each
EUR 248.4

Gibbs reagent

573630 1.0g
EUR 295

Lloyd Reagent

L04655 500G
EUR 307.17

Gibbs reagent

T7852-10mg 10mg Ask for price
Description: Gibbs reagent

Gibbs reagent

T7852-1g 1g Ask for price
Description: Gibbs reagent

Gibbs reagent

T7852-1mg 1mg Ask for price
Description: Gibbs reagent

Gibbs reagent

T7852-50mg 50mg Ask for price
Description: Gibbs reagent

Gibbs reagent

T7852-5mg 5mg Ask for price
Description: Gibbs reagent

Togni’s Reagent

T529000 10g
EUR 190

VeZol Reagent

R411-01 100 ml
EUR 76.4

VeZol Reagent

R411-02 200 ml
EUR 127.6

Tebbe's Reagent

T012880 10ml
EUR 339
Description: 67719-69-1

O’Meara Reagent

R031-100ML 1 unit
EUR 5.56
Description: O’Meara Reagent

DMACA Reagent

R035-10ML 1 unit
EUR 9.92
Description: DMACA Reagent

Folin's Reagent

ML059-125ML 1 unit
EUR 31.47
Description: Folin's Reagent

Folin's Reagent

ML059-250ML 1 unit
EUR 57.09
Description: Folin's Reagent

Schiff's Reagent

40120082-1 100 mL
EUR 35.14

Schiff's Reagent

40120082-2 500 mL
EUR 54.54

Bluing Reagent

BRT030 30 ml
EUR 7.72

Bluing Reagent

BRT125 125 ml
EUR 10.28

Bluing Reagent

BRT3800 1 Gal.
EUR 108

Bluing Reagent

BRT500 500 ml
EUR 20.58

Bluing Reagent

BRT999 1000 ml
EUR 30.85

Topfer Reagent

R021-100ML 1 unit
EUR 4.44
Description: Topfer Reagent

Millon's Reagent

R097-2.5L 1 unit
EUR 1266.85
Description: Millon's Reagent

Millon's Reagent

R097-250ML 1 unit
EUR 127.81
Description: Millon's Reagent

Millon's Reagent

R097-500ML 1 unit
EUR 228.41
Description: Millon's Reagent

Marfey’s reagent

RM10585-100MG 1 unit
EUR 229.58
Description: Marfey’s reagent

Biuret Reagent

ML122-100ML 1 unit
EUR 14.29
Description: Biuret Reagent

Biuret Reagent

ML122-500ML 1 unit
EUR 57.28
Description: Biuret Reagent

Chymase reagent

30C-CP1129 5 units Ask for price
Description: Purified native Human Chymase reagent

BURGESS REAGENT

702010 each Ask for price

FreeZol Reagent

R711-01 100 ml
EUR 71.2

FreeZol Reagent

R711-02 200 ml
EUR 127.2

Séroprevalence kapavky Toxoplasma u přežvýkavců pomocí latexového aglutinačního testu (LAT) a enzymového imunosorbentního testu (ELISA) v Assiut Governorate 

Toxoplasmosis is a vital zoonotic parasitic illness world-wide. Nonetheless, knowledge on Toxoplasma gondii an infection in ruminants are scarce in Assiut governorate, Egypt. Thus we carried out a survey on the seroprevalence of T. gondii an infection through the use of latex agglutination take a look at (LAT) and enzyme-linked immunosorbent assay (ELISA). A complete of 274 sera have been examined (56 imported camels, 56 cattle, 55 buffaloes, 50 sheep and 57 goats) within the interval from November 2014 – February 2016. The general seroprevalence in ruminants was (35.8%) by LAT and (83.6%) by ELISA. The very best charge of T. gondii an infection by LAT was present in goats (47.4%) adopted by sheep (44%), camels (35.7%), cattle (32.1%) and buffaloes (20%).

The very best charge of T. gondii an infection by ELISA was recorded in camels (96.4%) adopted by goats (87.7%), sheep (86%), buffaloes (74.5%) and cattle (73.2%). Antibody titres to T. gondii in optimistic sera by LAT ranged from 1:2 to 1:64 in imported camels, 1:2 to 1:32 in cattle, 1:2 to 1:Eight in buffaloes, from 1:Four to 1:64 in sheep and from 1:2 to 1:16 in goats. The variations of seroprevalence between totally different species have been vital by LAT and extremely vital by ELISA. Our outcomes counsel that the T. gondii parasite is broadly unfold on account of excessive publicity to infective cat faeces.

Detekce cytomegaloviru interleukinu 10 (cmvIL-10) pomocí enzymového imunoanalýzy (ELISA) 

Since its introduction in 1971, the enzyme-linked immunosorbent assay (ELISA) has revolutionized medication by enabling detection of each antigens and antibodies in a wide range of samples. We describe right here a personalized sandwich ELISA developed for the detection of Human Cytomegalovirus interleukin-10 (cmvIL-10). CmvIL-10 is a virally encoded cytokine and ortholog of human interleukin 10 (hIL-10).

Whereas cmvIL-10 and hIL-10 are related in construction and performance, total amino acid sequence id is just 27%, leading to antigenically distinct proteins. The cmvIL-10 ELISA is restricted and doesn’t detect hIL-10. The assay is delicate sufficient to detect cmvIL-10 in each tradition supernatants and affected person serum. The flexibility to quantify cmvIL-10 ranges throughout HCMV an infection might present precious details about immune evasion methods and viral management of host signaling pathways.

Vyhodnocení vazebné afinity ligandů E3 ubikvitin ligázy pomocí zapojení buněčného cíle a testu ELISA v buňce 

The invention of potent cell-permeable E3 ubiquitin ligase ligands can considerably facilitate the event of proteolysis focusing on chimeras (PROTACs). Right here, we current a protocol to find out the binding affinity of ligands towards CRBN E3 ubiquitin ligase, utilizing a mobile goal engagement mechanism and in-cell ELISA assay. This protocol is simple to ascertain, with comparatively low value and speedy timeframe. It will also be modified to measure the extent of different proteins or decide the ligand affinity towards different E3s. For full particulars on the use and execution of this protocol, please confer with Yang et al. (2020).

HODNOCENÍ VIZUÁLNÍHO IMUNOSORBENTNÍHO ROZBORU SPOJENÉHO S ENZYMY (ELISA) PRO ZJIŠTĚNÍ TEHOTNOSTI U ČTVRTÝCH DRUHŮ GULÁTŮ 

This research evaluated using a commercially accessible, visible enzyme-linked immunosorbent assay (ELISA) for analysis of being pregnant in okapi (Okapia johnstoni), gerenuk (Litocranius walleri), japanese big eland (Tragelaphus derbianus spp. gigas), and dama gazelle (Nanger dama). This assay has been validated to be used in home cattle, sheep, goats, and water buffalo. In contrast to different blood-based being pregnant related glycoprotein (PAG) detection strategies, this assay doesn’t require refined laboratory tools for detection or interpretation and may subsequently be utilized in lots of settings. Banked serum samples from gerenuk (n = 11), big eland (n = 4), dama gazelle (n = 33) and okapi (n = 3) have been examined, and a pregnant and nonpregnant pattern from every particular person have been included.

The ELISA confirmed 100% sensitivity and specificity in gerenuk and big eland samples, and 0% sensitivity in dama gazelle and okapi samples. Utilizing this assay, being pregnant was detected by 7-Eight wk gestation in gerenuk and 6 wk in big eland. These outcomes are according to earlier research that have been capable of precisely detect being pregnant in different family members Bovidae, however it’s potential that PAGs current in okapi and dama gazelle are structurally dissimilar relative to the meant take a look at goal, and are subsequently unrecognizable utilizing this take a look at.

The faint positivity within the dama gazelle assays could also be on account of cross-reactivity with different proteins within the pattern, or on account of inconsistent binding with the dama gazelle PAG. This ELISA seems to be an correct, speedy, and cheap methodology of point-of-care being pregnant analysis in gerenuk and big eland, however not okapi and dama gazelle. Extra research needs to be pursued to additional characterize the boundaries of being pregnant detection utilizing this assay in gerenuk and big eland, and to research the validity of this take a look at in different nondomestic ruminant species.

Napsat komentář

Vaše e-mailová adresa nebude zveřejněna. Vyžadované informace jsou označeny *