Fotooxidací indukovaný systém zesílení

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r-web

Fotooxidací indukovaný systém zesílení fluorescence pro ultracitlivý enzymový imunosorbentní test (ELISA) 

This report suggests a method of enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). The PIFA utilized autocatalytic photooxidation of the chemifluorescent substrate, 10-acetyl 3,7-dihydroxyphenoxazine (ADHP, Amplex Red) to amplify the fluorescent product resorufin, initially oxidized by horse radish peroxidase (HRP). As the amplification rate is proportional to the initial level of resorufin, the level of antigen labeled by HRP is quantified by analyzing the profile of fluorescence intensity.

The normalized profile was interpolated into an autocatalysis model, and the rate of increase at half-maximum time was quantified by the use of an amplification index (AI). The lower limit of detection, for resorufin or HRP, was less than one-tenth that of the plate reader. It requires only slight modification of the fluorescence reader and is fully compatible with conventional or commercial ELISA. When it is applied to a commercial ELISA kit for the detection of amyloid beta, it is verified that the PIFA assay enhanced the detection sensitivity by more than a factor of 10 and was compatible with a conventional 96-well ELISA assay kit. We anticipate this PIFA assay to be used in research for the detection of low levels of proteins and for the early diagnosis of various diseases with rare protein biomarkers, at ultra-low (pg/mL) concentrations.

r-web
r-web

Adiponectin (human) Elisa Assay Kit

K2192-96 96 assays
EUR 1017

Adiponectin (human) ELISA Assay Kit

K4901-100
EUR 898

Adiponectin (rat) Elisa Assay Kit

K4903-100
EUR 843

Mouse Adiponectin ELISA kit

LF-EK0239 1×96T
EUR 603

Mouse Adiponectin ELISA kit

LF-EK50601 1×96T
EUR 659

Mouse Adiponectin ELISA Kit

LF-EK70004 1×96T
EUR 528

Adiponectin ELISA Kit (Mouse)

55R-1412 96 assays
EUR 1326
Description: ELISA kit for detection of Adiponectin in the research laboratory

Mouse Adiponectin ELISA kit

55R-1721 1 kit
EUR 671
Description: ELISA kit for the detection of Adiponectin in the research laboratory

Rabbit Adiponectin (Adiponectin) ELISA Kit

abx051755-96tests 96 tests
EUR 793

Mouse Adipoq/ Adiponectin ELISA Kit

E0039Mo 1 Kit
EUR 546

Mouse adiponectin(ADP)ELISA Kit

GA-E0259MS-48T 48T
EUR 336

Mouse adiponectin(ADP)ELISA Kit

GA-E0259MS-96T 96T
EUR 534

Mouse Adiponectin (ADIPOQ) ELISA Kit

20-abx153600
  • EUR 5374.00
  • EUR 2868.00
  • EUR 676.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Adiponectin (ADIPOQ) ELISA Kit

abx575497-96tests 96 tests
EUR 637

Mouse Adiponectin PicoKine ELISA Kit

EK0596 96 wells
EUR 486
Description: For quantitative detection of mouse Adiponectin in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

Mouse Adiponectin (ADIPOQ) ELISA Kit

abx253954-96tests 96 tests
EUR 637

Mouse Adiponectin (ADP) ELISA Kit

DLR-ADP-Mu-48T 48T
EUR 317
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Adiponectin (ADP) ELISA Kit

DLR-ADP-Mu-96T 96T
EUR 398
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Adiponectin ELISA Kit (ADP)

RK02574 96 Tests
EUR 521

Mouse Adiponectin (ADP) ELISA Kit

RD-ADP-Mu-48Tests 48 Tests
EUR 295

Mouse Adiponectin (ADP) ELISA Kit

RD-ADP-Mu-96Tests 96 Tests
EUR 400

Mouse Adiponectin (ADP) ELISA Kit

RDR-ADP-Mu-48Tests 48 Tests
EUR 307

Mouse Adiponectin (ADP) ELISA Kit

RDR-ADP-Mu-96Tests 96 Tests
EUR 418

Adiponectin ELISA Kit (Mouse) (OKBB00292)

OKBB00292 96 Tests
EUR 583
Description: Description of target: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 10 pg/ml

ELISA kit for Mouse Adiponectin

EK2029 96 tests
EUR 469
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Adiponectin in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse adiponectin, ADP ELISA Kit

CSB-E07272m-24T 1 plate of 24 wells
EUR 165
Description: Quantitative sandwich ELISA kit for measuring Mouse adiponectin, ADP in samples from serum, plasma, cell culture supernates, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse adiponectin, ADP ELISA Kit

1-CSB-E07272m
  • EUR 602.00
  • EUR 4238.00
  • EUR 2256.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Mouse adiponectin, ADP in samples from serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Adiponectin (ADP) ELISA Kit

SEA605Mu-10x96wellstestplate 10x96-wells test plate
EUR 3213.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Adiponectin (ADP) ELISA Kit

SEA605Mu-1x48wellstestplate 1x48-wells test plate
EUR 352.25
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Adiponectin (ADP) ELISA Kit

SEA605Mu-1x96wellstestplate 1x96-wells test plate
EUR 460.36
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Adiponectin (ADP) ELISA Kit

SEA605Mu-5x96wellstestplate 5x96-wells test plate
EUR 1771.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Adiponectin (ADP) ELISA Kit

4-SEA605Mu
  • EUR 3264.00
  • EUR 1722.00
  • EUR 461.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Adiponectin (ADP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse adiponectin(ADP)ELISA Kit

QY-E20049 96T
EUR 361

Mouse Adiponectin ELISA kit (4X96T)

LF-EK0240 4×96T
EUR 2045

Mouse Adiponectin ELISA kit (10X96T)

LF-EK0241 10×96T
EUR 4484

Mouse Adiponectin ELISA kit (4X96T)

LF-EK50602 4×96T
EUR 2242

Mouse Adiponectin, Adipoq ELISA KIT

ELI-02177m 96 Tests
EUR 865

Mouse adiponectin,ADP ELISA Kit

CN-02830M1 96T
EUR 434

Mouse adiponectin,ADP ELISA Kit

CN-02830M2 48T
EUR 284

Mouse adiponectin,ADP ELISA kit

E03A1273-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse adiponectin,ADP ELISA kit

E03A1273-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse adiponectin,ADP ELISA kit

E03A1273-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Total Adiponectin ELISA kit

E03A0125-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Total Adiponectin ELISA kit

E03A0125-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Total Adiponectin ELISA kit

E03A0125-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Adiponectin ELISA Kit

55R-1411 96 assays
EUR 1340
Description: ELISA kit for detection of Adiponectin in the research laboratory

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 202

Human Adiponectin HMW( Adiponectin HMW) ELISA Kit

QY-E05586 96T
EUR 361

Adiponectin, Mouse

HY-P7129 50ug
EUR 418

ELISA kit for Mouse Adiponectin (ADP)

KTE70557-48T 48T
EUR 365
Description: Quantitative sandwich ELISA for measuring Mouse Adiponectin (ADP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Adiponectin (ADP)

KTE70557-5platesof96wells 5 plates of 96 wells
EUR 2525
Description: Quantitative sandwich ELISA for measuring Mouse Adiponectin (ADP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Adiponectin (ADP)

KTE70557-96T 96T
EUR 637
Description: Quantitative sandwich ELISA for measuring Mouse Adiponectin (ADP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse ADP (Adiponectin)

ELK1234 1 plate of 96 wells
EUR 372
Description: A sandwich ELISA kit for detection of Adiponectin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Adiponectin (ACRP30) AssayMax ELISA Kit

EMA2500-1 96 Well Plate
EUR 396

ELISA kit for Mouse adiponectin,ADP

EK0312 96 tests
EUR 712
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse adiponectin,ADP in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse ADP/Acrp30(Adiponectin) ELISA Kit

EM0001 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.469 ng/ml

Mouse Adiponectin Receptor 1 ELISA kit

E03A0524-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Adiponectin Receptor 1 ELISA kit

E03A0524-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Adiponectin Receptor 1 ELISA kit

E03A0524-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Adiponectin Receptor 2 ELISA kit

E03A0525-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Adiponectin Receptor 2 ELISA kit

E03A0525-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Adiponectin Receptor 2 ELISA kit

E03A0525-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Adiponectin Receptor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Cow Adiponectin (Adiponectin) CLIA Kit

20-abx491751
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Dog Adiponectin (Adiponectin) CLIA Kit

20-abx491752
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Chicken Adiponectin (Adiponectin) CLIA Kit

20-abx491753
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Pig Adiponectin (Adiponectin) CLIA Kit

20-abx491754
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Adiponectin (Adiponectin) CLIA Kit

20-abx491755
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rabbit Adiponectin (Adiponectin) CLIA Kit

20-abx491756
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Adiponectin ELISA Kit

MET-5052 96 assays
EUR 572

Human Adiponectin ELISA Kit

MET-5052-5 5 x 96 assays
EUR 2283

Human Adiponectin ELISA Kit

LF-EK0291 1×96T
EUR 603

Human Adiponectin ELISA kit

LF-EK50457 1×96T
EUR 659

Human Adiponectin ELISA Kit

LF-EK70001 1×96T
EUR 528

Rat Adiponectin ELISA Kit

LF-EK70005 1×96T
EUR 528

Adiponectin ELISA Kit (Rat)

55R-1413 96 assays
EUR 1291
Description: ELISA kit for detection of Adiponectin in the research laboratory

Human Adiponectin ELISA kit

55R-1720 1 kit
EUR 671
Description: ELISA kit for the detection of Adiponectin in the research laboratory

Human Adiponectin ELISA kit

55R-E09 96 wells
EUR 997
Description: ELISA kit for the detection of Adiponectin in the research laboratory

ADP/Acrp30 ELISA Kit| Mouse Adiponectin ELISA Kit

EF012820 96 Tests
EUR 689

Mouse Adiponectin PicoKine™ Fast ELISA Kit

FEK0596 96 wells
EUR 537
Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Adiponectin/ADIPOQ with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Adiponectin/ADIPOQ tissue specificity: Synthesized exclusively by adipocytes and secreted into plasma.

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

20-abx153601
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

abx575259-96tests 96 tests
EUR 707

ELISA kit for Mouse ADP/Acrp30 (Adiponectin)

E-EL-M0002 1 plate of 96 wells
EUR 377
Description: A sandwich ELISA kit for quantitative measurement of Mouse ADP/Acrp30 (Adiponectin) in samples from Serum, Plasma, Cell supernatant

Mouse Adiponectin Receptor 1(ADIPOR1)ELISA Kit

GA-E0863MS-48T 48T
EUR 336

Mouse Adiponectin Receptor 1(ADIPOR1)ELISA Kit

GA-E0863MS-96T 96T
EUR 534

Mouse Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

abx388529-96tests 96 tests
EUR 911

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

abx254739-96tests 96 tests
EUR 707

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

DLR-ADIPOR1-Mu-48T 48T
EUR 435
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Adiponectin Receptor 1 (ADIPOR1) in samples from serum, plasma or other biological fluids.

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

DLR-ADIPOR1-Mu-96T 96T
EUR 561
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Adiponectin Receptor 1 (ADIPOR1) in samples from serum, plasma or other biological fluids.

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

RD-ADIPOR1-Mu-48Tests 48 Tests
EUR 429

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

RD-ADIPOR1-Mu-96Tests 96 Tests
EUR 591

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

RDR-ADIPOR1-Mu-48Tests 48 Tests
EUR 447

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

RDR-ADIPOR1-Mu-96Tests 96 Tests
EUR 618

Mouse ADIPOR1(Adiponectin Receptor 1) ELISA Kit

EM0383 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 1.875 ng/ml

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

SEA147Mu-10x96wellstestplate 10x96-wells test plate
EUR 4391.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin Receptor 1 (ADIPOR1) in serum, plasma and other biological fluids.

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

SEA147Mu-1x48wellstestplate 1x48-wells test plate
EUR 449.27
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin Receptor 1 (ADIPOR1) in serum, plasma and other biological fluids.

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

SEA147Mu-1x96wellstestplate 1x96-wells test plate
EUR 598.96
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin Receptor 1 (ADIPOR1) in serum, plasma and other biological fluids.

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

SEA147Mu-5x96wellstestplate 5x96-wells test plate
EUR 2395.32
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin Receptor 1 (ADIPOR1) in serum, plasma and other biological fluids.

Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

4-SEA147Mu
  • EUR 4442.00
  • EUR 2346.00
  • EUR 599.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Adiponectin Receptor 1 (ADIPOR1) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

Prevalence strongyloidiázy v kohortě migrantů v Itálii a přesnost nového testu ELISA pro S. stercoralis Infekce, průřezová studie 

Strongyloides stercoralis infection is a life-threatening neglected tropical disease. Diagnostic issues have caused an underestimation of its global burden. The choice of appropriate diagnostic tests for the screening of populations at risk of the infection, such as migrants from endemic countries, is of paramount importance. From November 2017 to July 2018, all migrants presenting to the National Institute for Health Migration and Poverty (INMP) in Rome, Italy were offered screening tests for S. stercoralis infection. The study objective was to estimate the prevalence of strongyloidiasis in the study population and the accuracy of a novel ELISA assay.

The following tests were carried out at the IRCCS Sacro Cuore Don Calabria hospital in Negrar, Verona: stool microscopy, real-time PCR for S. stercoralis, in-house immunofluorescence test (IFAT), a commercial ELISA assay (Bordier ELISA), and a novel ELISA assay (Euroimmun ELISA). A latent class analysis (LCA) model set up with test results, clinical variables, and eosinophilia indicated a prevalence around 7.5%, in line with previous findings. The sensitivity and the specificity of Euroimmun ELISA were 90.6% (95% CI 80.5-100) and 87.7% (95CI 84.5-91.0); these results indicate that the novel ELISA assay would be suitable for screening of migrants from endemic countries.

Analýza funkčních virů generovaných PAMP RNA pomocí testu ELISA IFNα / β 

Virus-generated PAMP RNAs are key factors that activate host immune response. The PAMP RNAs are therefore usually closely related with viral disease pathogenesis. Quantitative real time PCR is a conventional method to assess RNA. However, it cannot be used for detecting short dsRNAs generated by viral replicase. This protocol was established to analyze the PAMP RNAs produced by viruses which are able to induce host immune response. Classical viral PAMP RNAs and non-classical viral PAMP RNAs are analyzed separately.

Briefly, to access total viral PAMP RNAs, total RNA was extracted from the virus infected cells and then transfected into Cop5 cells. Whereas, to assess non-classical viral PAMP RNAs, the constructs expressing viral replicase are transfected into Cop5 cells. The amount of IFNα/β produced by Cop5 cells, determined by ELISA, is correlated with the total and non-classical viral PAMP RNAs. Since this method is based on type I IFN response, it is therefore suitable for measuring the functional virus-generated PAMP RNAs and also for assessing the efficiency of these PAMP RNAs.

Vývoj sendviče s dvojitou protilátkou a enzymovým imunosorbentem (DAS-ELISA) pro detekci KHV 

Koi herpesvirus disease (KHVD) caused by the koi herpesvirus (KHV) is difficult to diagnose in live fish, presenting a challenge to the koi industry. The enzyme-linked immunosorbent assay (ELISA) method cannot be widely used to detect KHV because few commercial anti-KHV antibody exists. Here, we developed an anti-ORF132 polyclonal antibody and confirmed its reactivity via indirect immunofluorescence assay and Western blotting. A double-antibody sandwich ELISA (DAS-ELISA) was established to detect KHV, monoclonal antibody 1B71B4 against ORF92 was used as the capture antibody, and the detection antibody was the polyclonal antibody against the truncated ORF132.

The lowest limit was 1.56 ng/ml KHV. Furthermore, the DAS-ELISA reacted with KHV isolates, while no cross-reactions occurred with carp oedema virus, spring viraemia of carp virus, frog virus 3 and grass carp reovirus. Two hundred koi serum samples from Guangdong, China, were used in the DAS-ELISA test, and the positive rate of the koi sera was 13%. The clinical sensitivity and specificity of the DAS-ELISA relative to the traditional PCR method were 66.7% and 97.6%, respectively. Our findings may be useful for diagnosing and preventing KHVD in koi and common carp.

Enzymově vázaný imunosorbentní test (ELISA) s použitím rekombinantního Fasciola katepsinu L1 pro diagnostiku lidské fasciolózy způsobené hybridním typem Fasciola hepatica / gigantica

Recombinant Fasciola cathepsin L-1 (rCatL1) was evaluated in enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human fasciolosis in Japan. Quality characteristics of the test were accessed by receiver operating characteristic (ROC) analysis, with sera from fasciolosis patients (n = 10), patients with no evidence of parasitic infections (n = 29), and patients with other helminth infections (n = 119). Both the sensitivity and specificity of the test achieved 100% with the control samples. To test the performance of the assay in an authentic situation, 311 serum samples, which had been sent to our laboratory for the diagnosis of parasitic infections from January 2018 to February 2019, were re-assessed using the rCatL1 ELISA. In this case, the sensitivity of the rCatL1 ELISA was 100%, giving positive results to all fasciolosis sera (n = 7), and the specificity was 99.0%, in which three of the 304 non-fasciolosis samples were judged positive.

Careful re-examination of the laboratory data and medical imaging of these three patients revealed that one of the patients, who had been diagnosed as having larva migrans syndrome, was judged to be infected with Fasciola, in addition to ascarid nematodes. Thus the true specificity of the assay in the authentic reached 99.3% (302/304). As the rCatL1 ELISA exhibited a highly significant positive likelihood ratio (152.0) and negative likelihood ratio (0.0), calculated from the 311 sample data, this rCatL1 ELISA can be used for routine screening and definitive diagnosis test for fasciolosis in reference laboratories.

Plasma Sample Diluent (Antigen-Down)-SD2

KF17353 500 ml
EUR 282

Human CA125 Calibrator Grade Protein

abx060961-50kU 50 kU
EUR 481

Human CA242 Calibrator Grade Protein

abx060974-50kU 50 kU
EUR 1156

Human CA50 Calibrator Grade Protein

abx060975-50kU 50 kU
EUR 1567

CA19-9 Calibrator Grade Protein

abx061026-50kU 50 kU
EUR 523

CA19-9 Calibrator Grade Protein

abx061027-50kU 50 kU
EUR 523

Recombinant CEA Protein (Calibrator Grade)

VAng-Wyb8648-inquire inquire Ask for price
Description: Carcinoembryonic Antigen, Calibrator Grade, recombinant protein from Cell Culture, 2.23 mg/mL.

BSA Calibrator [Bradford Assay Kit]

0209C 0.5 ml
EUR 103

Human Serum calibrator 3 (2X)

35-S63010000-L4 10 ml
EUR 127
Description: Human Serum calibrator 3 (2X)

Human Serum calibrator 3 (2X)

35-S7703H000-L3 10 ml
EUR 122
Description: Human Serum calibrator 3 (2X)

Cardiac Troponin I Calibrator Set

35R-AT01 set
EUR 1272
Description: Human Cardiac Troponin I Calibrator/Standard Set

HBV Surface Antigen (Subtype ad) (Human Plasma)

VAng-Lsx04581-1mg 1 mg
EUR 531
Description: Hepatitis B Surface Antigen (HBsAg-subtype Ad from Human Plasma.

Troponin I Cardiac Calibrator Set Protein

abx060754-1Set 1 Set
EUR 2054

Human Lipoprotein (a) Calibrator 3 Protein

abx060886-5ml 5 ml
EUR 342

Human CA125 LCR Calibrator Grade Protein

abx060963-50kU 50 kU
EUR 718

Human CA125 LCR Calibrator Grade Protein

abx060964-50kU 50 kU
EUR 718

Human CA125 LCR Calibrator Grade Protein

abx060971-50kU 50 kU
EUR 718

Recombinant HBV Surface Antigen (Subtype ad) (Human Plasma)

VAng-Wyb8581-inquire inquire Ask for price
Description: Hepatitis B Surface Antigen (HBsAg-subtype ad), recombinant protein from human plasma.

Human Apolipoprotein AI / B Calibrator 3 Protein

abx060868-10ml 10 ml
EUR 676

Human CA15-3 LCR Calibrator Grade Protein

abx060966-50kU 50 kU
EUR 1511

ExoStep Plasma

ExoS-25-P81 25 test
EUR 550.5

Donkey Plasma

88R-D002 100 ml
EUR 111
Description: Control Donkey Plasma

EDTA plasma

90-1009 100 ml
EUR 2028
Description: BNP and NT-proBNP free plasma EDTA

Hepatitis Surface Antigen subtype Ad (HBsAg Ad), Native (human plasma) purified

HBA26-N-100 100 ug
EUR 225

Hepatitis Surface Antigen subtype Ay (HBsAg Ay), Native (human plasma) purified

HBA28-N-100 100 ug
EUR 225

De-Plasma™

D022-1x25MG 1 x 25 mg
EUR 77

De-Plasma™

D022-5x25MG 5 x 25 mg
EUR 127

Dog Plasma (Heparin)

abx098253-50ml 50 ml
EUR 495

Human Plasma Fibronectin

FB001 1 mg
EUR 466

Chylomicrons, Human Plasma

CMIC15-N 1 mg
EUR 347

Transferrin, Human Plasma

TF16-N 10 mg
EUR 164

Apotransferrin, Human Plasma

TF25-N-10 10 mg
EUR 164

ExoStepTM Plasma + Standard

ExoS-25-PST81 25 test
EUR 726

Kallikrein, Human Plasma

KAL15-N-50 50 ug
EUR 286

Hemopexin, Human Plasma

HPEX15-N-100 100 ug
EUR 164

Angiotensinogen, Human Plasma

ATN15-N-100 100 ug
EUR 225

Vitronectin, Human Plasma

4087-100
EUR 457

Plasmin, Human Plasma

4089-1000
EUR 272

Kallikrein, Human Plasma

4093-50
EUR 272

Ceruloplasmin, Human Plasma

4096-1000
EUR 229

Apotransferrin, Human Plasma

4707-100
EUR 169

Chylomicrons, Human Plasma

7285-1000
EUR 501

Apotransferrin, Mouse Plasma

7540-1000
EUR 272

Apotransferrin, Rat Plasma

7541-1000
EUR 370

Transferrin, Rat Plasma

7543-1000
EUR 251

Hemopexin, Human Plasma

7544-1000
EUR 572

Albumin, Human Plasma

7546-1
EUR 240

Plasminogen, Human Plasma

7549-1
EUR 283

Prothrombin, Human Plasma

7684-1
EUR 180

Prekallikrein, Human Plasma

7685-100
EUR 224

Ceruloplasmin, Human Plasma

P1452-1
EUR 479

Rabbit Plasma (EDTA)

88R-1029E 500 ml
EUR 230
Description: Sterile Rabbit Plasma with EDTA

Lithium Heparin Plasma

90-1016 100 ml
EUR 370
Description: Lithium Heparin Human Plasma Sterile filtered

Human Plasma Renin Activity(Plasma Renin Activity) ELISA Kit

QY-E05530 96T
EUR 361

De-Plasma™ II

D030-10MG 10 mg
EUR 152

De-Plasma™ II

D030-5x10MG 5 x 10 mg
EUR 453

Anti-Bovine Plasma Proteins

BPP04 1 ml
EUR 334
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur.

Anti-Bovine Plasma Proteins

BPP04-AF 1 mg
EUR 1913
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur.

Anti-Bovine Plasma Proteins

BPP04-PA 1 ml
EUR 488
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur.

Anti-Bovine Plasma Proteins

BPP04AF-HRP 100 ul
EUR 989
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur.

Human Plasma Ceruloplasmin Protein

abx060936-1mg 1 mg
EUR 370

Human Plasma Haptoglobin Protein

abx060943-5mg 5 mg
EUR 565

Human Plasma Kallikrein Protein

abx060944-005mg 0.05 mg
EUR 356

Human Plasma Transferrin Protein

abx060950-100mg 100 mg
EUR 370

Seminal Plasma, Human Protein

abx069938-10ml 10 ml
EUR 926

Thrombin from bovine plasma

abx082464-1000U 1000 U
EUR 230

Normal Feline Plasma (EDTA)

abx098254-50ml 50 ml
EUR 704

Normal Feline Plasma (Heparin)

abx098255-50ml 50 ml
EUR 732

Plasma Prekallikrein (KLKB1) Antibody

20-abx004066
  • EUR 411.00
  • EUR 105.00
  • EUR 592.00
  • EUR 182.00
  • 100 ul
  • 10 ul
  • 200 ul
  • 50 ul

Plasma Prekallikrein (KLKB1) Antibody

abx027801-400ul 400 ul
EUR 523

Plasma Prekallikrein (KLKB1) Antibody

abx027801-80l 80 µl
EUR 286

Lactoferrin (Seminal Plasma) Protein

20-abx262658
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Plasma Prekallikrein (KLKB1) Antibody

20-abx318753
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Plasma Prekallikrein (Klkb1) Antibody

20-abx319651
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Plasma Prekallikrein (KLKB1) Antibody

20-abx338997
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Plasma Prekallikrein (KLKB1) Antibody

abx145872-100ug 100 ug
EUR 391

Plasma Kallikrein HC Antibody

20-abx141274
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Anti-Plasma Fibronectin antibody

STJ16100801 100 µg
EUR 506

Human IgG1, Myeloma Plasma

20007-G1-1 1 mg
EUR 202

Human IgG2, Myeloma Plasma

20007-G2-1 1 mg
EUR 202

Human IgG3, Myeloma Plasma

20007-G3-1 1 mg
EUR 408

Human IgG3, Native Plasma

20007-G3-1P 100 ug
EUR 347

Human IgG4, Myeloma Plasma

20007-G4-500 500 ug
EUR 225

Plasma kallikrein Polyclonal Antibody

42232-100ul 100ul
EUR 333

Plasma Prekallikrein (KLKB1) Antibody

20-abx324441
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Human Plasma kallikrein (KLKB1)

1-CSB-YP012461HU
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Plasma kallikrein(KLKB1),partial expressed in Yeast

Rat Plasma kallikrein (Klkb1)

1-CSB-YP012461RA
  • EUR 504.00
  • EUR 265.00
  • EUR 1832.00
  • EUR 763.00
  • EUR 1216.00
  • EUR 334.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Rat Plasma kallikrein(Klkb1),partial expressed in Yeast

Rat Plasma kallikrein (Klkb1)

1-CSB-EP012461RA
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Rat Plasma kallikrein(Klkb1),partial expressed in E.coli

Plasmin, Human Plasma, Lyophilized

PLN25-N 1 mg
EUR 225

Kininogen, HMW, Human Plasma

KINH15-N-50 50 ug
EUR 225

Kininogen, LMW, Human Plasma

KINL15-N-50 50 ug
EUR 225

Apolipoprotein J, Human Plasma

APOJ15-N-10 10 ug
EUR 286

Human Plasma, 0.1um Filtered

AK9577-0100 100mL Ask for price

Factor Va, Human Plasma

4098-10
EUR 283

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