Srovnání testů vazebné aktivity na destičkový faktor

0 Comments
r-web

Srovnání testů vazebné aktivity na destičkový faktor von Willebrandova: ELISA překrývá typ 2B se ztrátou multimerů HMW  Background: Plenty of new assays with completely different measuring ideas are out there to measure VWF GPIb-binding exercise, however little is understood about how these assays would possibly behave otherwise for subtypes of VWD. Targets: The COMPASS-VWF examine was designed […]

Vyhodnocení ELISA, imunofluorescence a stanovení

0 Comments
r-web

Vyhodnocení ELISA, imunofluorescence a stanovení laterálního průtoku pro SARS-CoV-2 sérologické testy  Background: The SARS-CoV-2 outbreak has emerged at the end of 2019. Aside from the detection of viral genome with specific RT-PCR, there is a growing need for reliable determination of the serological status. We aimed at evaluating five SARS-CoV-2 serology assays. Methods: An in-house immunofluorescence assay […]

Fotooxidací indukovaný systém zesílení

0 Comments
r-web

Fotooxidací indukovaný systém zesílení fluorescence pro ultracitlivý enzymový imunosorbentní test (ELISA)  This report suggests a method of enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). The PIFA utilized autocatalytic photooxidation of the chemifluorescent substrate, 10-acetyl 3,7-dihydroxyphenoxazine (ADHP, Amplex Red) to amplify the fluorescent product resorufin, initially oxidized by horse radish peroxidase (HRP). […]

Neočekávané falešně pozitivní sazby

0 Comments
r-web

Neočekávané falešně pozitivní sazby v pediatrické sérologii SARS-CoV-2 s použitím testu ELIM IgG Anti-SARS-CoV-2 ELISA IgG   Aims: Serologic assay efficiency research for extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-​2) in pediatric populations are missing, and few seroprevalence research have routinely included orthogonal testing to enhance accuracy. Strategies: Remnant serum samples for routine bloodwork from 2,338 pediatric […]

Dopad komplementu a rozdíl buněčného testu a ELISA

0 Comments
r-web

Dopad komplementu a rozdíl buněčného testu a ELISA při stanovení neutralizační kapacity proti viru příušnic a spalniček  Neutralizing antibodies in opposition to mumps and measles virus are thought-about a correlate of safety in opposition to these ailments. Measurement of neutralizing antibodies is usually carried out utilizing plaque discount neutralization assay or 50% cell tradition infective […]

Srovnání myší Toxoplasma gondii

0 Comments
r-web

Srovnání myší Toxoplasma gondii a antigenů odvozených z buněčné kultury v testu ELISA   Toxoplasmosis is an infectious illness brought on by the coccidian parasite Toxoplasma gondii. Prognosis relies on serological strategies with detection of particular IgG and IgM antibodies. The current research was carried out to check the sensitivity and specificity of soluble antigen […]